HPLC-ELSD检测方法对神经酰胺混合物的检测分析

HPLC condition

Sample: Ceramide mixture (about 500 ng each)

Column: 3499拉斯维加斯色谱柱91181545 C18（4.6mm ID Ｘ150mm L）

Column temp: 35°C

mobiles phase: A: Chloroform  B: 5％H2O/MeOH

Gradient: 0min→1min→10min、7 ％B→7 ％B→50％B

Flow rate: 1.2ml/min

SofTA ELSD Conditions: SC=45°C,DT= 55°C,Filter=3,Gain=Normal